ProHiFlo: Hierarchical Flow Matching with Functional Guidance for De Novo Protein Generation

In three linesProHiFlo is a hierarchical flow matching framework for de novo protein generation. It combines coarse-to-fine generation (backbone then atoms), functional guidance via pretrained predictors, and SE(3)-equivariant architecture. On enzyme active site scaffolding, ProHiFlo achieves 58.9% success rate vs 41.2% for RFDiffusion, with 4× fewer sampling steps.

Summary generated by Claude — human-verified